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Cloning Reagents
for Every Construct

  • Exceptional accuracy minimizes screening and saves time
  • Unique mechanism prevents junction errors and empty vector background
  • Unparalleled versatility for a variety of applications
  • Quick, scalable workflow that can be easily automated
Seamless cloning
Ligation independent
No need for subcloning
Site-directed mutagenesis

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In-Fusion Snap Assembly Vs GeneArt Gibson Assembly in multi- and large-fragment assemblies            

In-Fusion Snap Assembly from Takara Bio uses a seamless cloning-based strategy, which smoothly places DNA fragments of any size or sequence into the destination vector without leaving scars. The simple and elegant technology makes it easy to carry out the most complicated types of cloning experiments, such as simultaneous insertion of multiple fragments or the insertion of a large fragment in just fifteen minutes.

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The right clone, every time
3 simple steps to seamless & accurate cloning

The In-Fusion Cloning products allow ligation-independent cloning of PCR products into any vector, at any site of linearization, to which takes as little as 15 minutes.


It is specific and directional, ensuring an exceptionally high rate of cloning accuracy in all applications.

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Takara Bio is committed to providing best-in-class products that empower researchers to overcome technical hurdles and focus on the advancement of scientific knowledge.

Note: Rating is based on over 5000 citations for In-Fusion Cloning on Bioz

One product, multiple application

In-Fusion Cloning is beautifully versatile. With an easy-to-follow protocol that provides high efficiency, you can successfully perform a wide array of applications, all with the same kit. Clone your insert specifically and directionally, and get an exceptionally high rate of cloning accuracy in every experiment.

Cloning shouldn't be work;
it should just work!

A beautiful & simplified workflow with
Takara Bio In-Fusion Cloning

The homologous ends between insert(s) and vector are designed into your primers, and the two are annealed during the In-Fusion Cloning reaction. Because there is no need to digest the insert(s) prior to incubation with the vector, the In-Fusion Cloning workflow is significantly shorter than that of traditional cloning by restriction enzymes.


This technology does not require a ligation step either, saving even more time and getting you to the transformation step sooner. With empty vector background levels approaching zero, you can pick just a few colonies and be confident that you have the right clone.

Know more about In-Fusion Cloning

In-Fusion Cloning Mechanism

Clone any insert, with any vector, at any site. In-Fusion seamless cloning technology makes it easy! Here’s how.

Applications using In-Fusion Cloning

In-Fusion Cloning is beautifully versatile—learn about the different ways you can put it to work.

In-Fusion Cloning vs other seamless cloning technologies

In-Fusion Cloning is the most elegant and simple seamless cloning technology available. Learn why.

More videos at Learning Center

Design your primers

Design primers for single- or multi-insert cloning or for your site-directed mutagenesis experiment (insertion, deletion, replacement) with our primer design tool.
Try It Yourself

​Let us help you find the best for your project.

Note: Rating is based on over 5000 citations for In-Fusion Cloning on Bioz

Romita Thounaojam, PhD

Product Manager — Cloning

DSS Takara Bio India Pvt Ltd

ON DEMAND WEBINAR

In-Fusion Cloning : Seamless Way To Overcome Cloning Challenges by Takara Bio Kits 



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